[CAS NO. 1415246-68-2] MMAFhydrochloride

Name: MMAFhydrochloride
Brand: Arctom Scientific
SKU: HY-15579A

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PRODUCTS SPECIFICATIONS [1415246-68-2]

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Catalog

HY-15579A

Brand

MCE

CAS

1415246-68-2

DESCRIPTION [1415246-68-2]

Overview

MDL	-
Molecular Weight	768.42
Molecular Formula	C39H66ClN5O8
SMILES	CC(C)[C@H](NC)C(N[C@H](C(N([C@@H]([C@@H](C)CC)[C@H](OC)CC(N1CCC[C@@]1([H])[C@H](OC)[C@@H](C)C(N[C@H](C(O)=O)CC2=CC=CC=C2)=O)=O)C)=O)C(C)C)=O.Cl

For research use only. We do not sell to patients.

7 Publications Citing Use of MCE

Summary

MMAF (Monomethylauristatin F) hydrochloride is a potent tubulin polymerization inhibitor and is used as a antitumor agent. MMAF hydrochloride is widely used as a cytotoxic component of antibody-drug conjugates ( ADC s) such as Vorsetuzumab mafodotin and SGN-CD19A ^[1] ^[2] ^[3] .

IC50 & Target

Auristatin

In Vitro

MMAF inhibits anaplastic large cell lymphoma Karpas 299, breast carcinoma H3396, renal cell carcinoma 786-O and Caki-1 cells with IC ₅₀ s of 119, 105, 257 and 200 nM in vitro cytotoxicity assay ^[4] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF ₄ has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF ₄ ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively ^[4] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

* The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

DMSO : 27 mg/mL ( 35.14 mM ; Need ultrasonic and warming)

H ₂ O : < 0.1 mg/mL (ultrasonic;warming;heat to 60°C) (insoluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3014 mL	6.5069 mL	13.0137 mL
5 mM	0.2603 mL	1.3014 mL	2.6027 mL
10 mM	0.1301 mL	0.6507 mL	1.3014 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution
2.

Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)

Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution
3.

Add each solvent one by one: 10% DMSO >> 90% corn oil

Solubility: ≥ 2.5 mg/mL (3.25 mM); Clear solution

* All of the co-solvents are available by MCE.