[CAS NO. 1477-57-2] Win 18446

Name: Win 18446
Brand: Arctom Scientific
SKU: HY-W011094

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PRODUCTS SPECIFICATIONS [1477-57-2]

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Catalog

HY-W011094

Brand

MCE

CAS

1477-57-2

DESCRIPTION [1477-57-2]

Overview

MDL	MFCD00000841
Molecular Weight	366.11
Molecular Formula	C12H20Cl4N2O2
SMILES	O=C(NCCCCCCCCNC(C(Cl)Cl)=O)C(Cl)Cl

For research use only. We do not sell to patients.

1 Publications Citing Use of MCE

[1]Sci Adv. 2020 Nov 4;6(45):eaaz1410.

Summary

Win 18446 is an orally active testes-specific enzyme ALDH1a2 inhibitor, with an IC ₅₀ of 0.3 μM. Win 18446 reversibly inhibits spermatogenesis in many species and inhibits Retinoic acid (HY-14649) biosynthesis from Retinol (HY-B1342) within the testes ^[1] ^[2] .

In Vitro

WIN 18446 (1 μM, 24 h) is able to suppress Stra8 expression in whole-testes cultures that contained both germ and somatic cells ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR ^[2]

Cell Line:	Testes were collected from 2-dpp mice.
Concentration:	1 μM.
Incubation Time:	24 h.
Result:	Results in a VAD-like phenotype in the testis. Alone or in combination with ROL significantly reduced Stra8 expression.

In Vivo

Win 18446 (200 mg/kg, Orally for 4, 8, and 16 weeks) significantly reduces intratesticular concentrations of retinoic acid, severely impaired spermatogenesis, and caused infertility ^[1] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-month-old male New Zealand white rabbits ^[1] .
Dosage:	200 mg/kg.
Administration:	Orally for 4, 8, and 16 weeks.
Result:	Resulted in marked suppression of testicular weight over time. Dramatically suppressed spermatogenesis. Significantly reduces intratesticular concentrations of retinoic acid and Stra8 expression and very severely suppresses spermatogenesis and fertility.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL ( 273.14 mM ; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7314 mL	13.6571 mL	27.3142 mL
5 mM	0.5463 mL	2.7314 mL	5.4628 mL
10 mM	0.2731 mL	1.3657 mL	2.7314 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% DMSO >> 40% PEG300 >> 5% Tween-80 >> 45% saline

Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

* All of the co-solvents are available by MCE.

References

Synonyms

Acetamide, N,N′-1,8-octanediylbis[2,2-dichloro-

Acetamide, N,N′-octamethylenebis[2,2-dichloro-

N,N′-1,8-Octanediylbis[2,2-dichloroacetamide]

Win 18446

N,N′-Bis(dichloroacetyl)-1,8-octamethylenediamine

Win 18441

N,N′-Octamethylenebis(2,2-dichloroacetamide)

N,N′-Bis(dichloroacetyl)-1,8-diaminooctane

Fertilysin

Bis-Diamine

Fertilysine

NSC 59354

2,2-Dichloro-N-[8-[(2,2-dichloroacetyl)amino]octyl]acetamide