[CAS NO. 23109-05-9] α-Amanitin

Name: α-Amanitin
Brand: Arctom Scientific
SKU: HY-19610

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PRODUCTS SPECIFICATIONS [23109-05-9]

Store

Catalog

HY-19610

Brand

MCE

CAS

23109-05-9

DESCRIPTION [23109-05-9]

Overview

MDL	-
Molecular Weight	918.97
Molecular Formula	C39H54N10O14S
SMILES	O=[S@](C[C@@](NC(CNC([C@@]([C@@H](C)CC)([H])N1)=O)=O)([H])C(N[C@@](C(N(C[C@H](O)C2)[C@]2([H])C3=O)=O)([H])CC(N)=O)=O)C4=C(C[C@](C(NCC1=O)=O)([H])NC([C@](N3)([H])[C@@H](C)[C@@H](O)CO)=O)C5=CC=C(O)C=C5N4

For research use only. We do not sell to patients.

19 Publications Citing Use of MCE

Summary

α-Amanitin is the principal toxin of several deadly poisonous mushrooms, exerting its toxic function by inhibiting RNA-polymerase II .

IC50 & Target

Traditional Cytotoxic Agents

In Vitro

α-Amanitin decreases TAF15 mRNA and TAF15 protein levels in MKN45 cells, and inhibits the RNAPII activity towards TAF15 mRNA ^[2] . alpha-Amanitin decreases cell viability by 14%, 21%, 41%, 44%, and 50% at concentrations of 100, 10, 1, 0.1, and 0.01 µg/mL, respectively. The LD ₅₀ of the alpha-Amanitin at 36 h is measured as 1 µg/mL. The total amount of protein within the cell at 24 h is significantly increased for the 1 µg/mL dose of alpha-Amanitin compared to the control ^[3] . α-Amanitin dramatically decreases the expression of gap junctional genes (Gja1, Gja4 and Gjc1) and FSHr and LHr in cumulus cells ^[4] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The intravenous LD ₅₀ dose of alpha-Amanitin is 0.327 mg/kg body weight after intravenous injection into BALB/c mice. After 12 h of alpha-Amanitin injection in caudal vein, the levels of WBC, RBC and HGB decrease significantly, while those of BUN and Crea increase significantly in serum. alpha-Amanitin inhibits some genes (Hsp90b1, Irx4, etc.), whose encoded proteins regulate the RNA polymerase II activity. alpha-Amanitin down-regulates some proteins (Nmi, Trpc5, etc.) taking part in the transcription progress ^[1] . alpha-Amanitin has potent activity in DTC suppression. Mice injected with alpha-Amanitin (0.4 mg/kg, i.p.)-treated cells maintain their body weight, while those receiving a peritoneal injection of MKN45 cells show a constant decrease in body weight ^[2] .

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Appearance

Solid

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

* The compound is unstable in solutions, freshly prepared is recommended.

Solvent & Solubility

In Vitro:

H ₂ O : 100 mg/mL ( 108.82 mM ; Need ultrasonic)

DMSO : 50 mg/mL ( 54.41 mM ; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.0882 mL	5.4409 mL	10.8817 mL
5 mM	0.2176 mL	1.0882 mL	2.1763 mL
10 mM	0.1088 mL	0.5441 mL	1.0882 mL

* Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: PBS

Solubility: 25 mg/mL (27.20 mM); Clear solution; Need ultrasonic and warming and heat to 60°C

* All of the co-solvents are available by MCE.