[CAS NO. 36913-39-0] MPP+ iodide
PRODUCTS SPECIFICATIONS [36913-39-0]
DESCRIPTION [36913-39-0]
Overview
| MDL | MFCD00055096 |
|---|---|
| Molecular Weight | 297.14 |
| Molecular Formula | C12H12IN |
| SMILES | C[N+]1=CC=C(C2=CC=CC=C2)C=C1.[I-] |
3 Publications Citing Use of MCE
Summary
MPP+ iodide, a toxic metabolite of the neurotoxin MPTP, causes symptom of Parkinson's disease in animal models by selectively destroying dopaminergic neurons in substantia nigra. MPP+ iodide is taken up by the dopamine transporter into dopaminergic neurons where it exerts its neurotoxic action on mitochondria by affecting complex I of the respiratory chain. MPP+ iodide is also a high affinity substrate for the serotonin transporter (SERT) [1] [2] .
In Vitro
MPP+ (1-3 mM; 24 hours) remarkably decreases the viability of cells
[1]
.
MPP+ also inhibits the activity of nicotinamide adenosine dinucleotide (NADH)-linked respiration in mitochondrial preparations and impairs aerobic glycolysis, leading to the present belief that the inhibition of NADH-linked cell respiration may constitute the final molecular mechanism of MPP+ neurotoxicity
[3]
.
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | SH-SY5Y cells |
| Concentration: | 1, 2, 3 mM |
| Incubation Time: | 24 hours |
| Result: | SH-SY5Y cells were treated with MPP+, mimicking the progress of dopaminergic neurons loss in PD; Reduced cell viability in both dose-dependent (1, 2, 3 mM for 24 h) and time dependent (1 mM) manner. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
H 2 O : 100 mg/mL ( 336.54 mM ; Need ultrasonic)
DMSO : 62.5 mg/mL ( 210.34 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 3.3654 mL | 16.8271 mL | 33.6542 mL |
| 5 mM | 0.6731 mL | 3.3654 mL | 6.7308 mL |
| 10 mM | 0.3365 mL | 1.6827 mL | 3.3654 mL |
-
1.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (336.54 mM); Clear solution; Need ultrasonic
-
2.
Add each solvent one by one: 10% DMSO >> 90% corn oil
Solubility: 2.5 mg/mL (8.41 mM); Suspended solution; Need ultrasonic
-
3.
-
4.
Add each solvent one by one: 10% DMSO >> 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (7.00 mM); Clear solution
References
- [1] Zhao M, et al. Mitochondrial calcium dysfunction contributes to autophagic cell death induced by MPP+ via AMPK pathway. Biochem Biophys Res Commun. 2019;509(2):390-394.
- [2] Martí Y, et al. Methyl-4-phenylpyridinium (MPP+) differentially affects monoamine release and re-uptake in murine embryonic stem cell-derived dopaminergic and serotonergic neurons. Mol Cell Neurosci. 2017;83:37-45.
- [3] Charlton CG. 1-Methyl-4-phenylpyridinium (MPP+) but not 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) serves as methyl donor for dopamine: a possible mechanism of action. J Geriatr Psychiatry Neurol. 1992;5(2):114-118.
Synonyms
