[CAS NO. 615538-51-7] DC0-NH2
PRODUCTS SPECIFICATIONS [615538-51-7]
DESCRIPTION [615538-51-7]
Overview
| MDL | - |
|---|---|
| Molecular Weight | 550.01 |
| Molecular Formula | C31H24ClN5O3 |
| SMILES | O=C(C(N1)=CC2=C1C=CC(N)=C2)NC3=CC4=C(NC(C(N5C[C@@H](CCl)C6=C5C=C(O)C7=CC=CC=C67)=O)=C4)C=C3 |
Summary
DC0-NH2 is an effector moiety for ADC and a simplified analog of DC1 with better stability. DC0-NH2 is about 1000-fold more cytotoxic than commonly used anticancer drugs (ex. Doxorubicin). DC0-NH2 can bind to the minor groove of DNA, followed by alkylation of adenine residues by its propabenzindole (CBI) component [1] .
IC50 & Target
|
Duocarmycins |
In Vitro
DC0-NH2 (0-3 nM; 72 hours) is highly potent against Ramos, Namalwa, and HL60/s cells with IC 50 values in the 1 pM to 10 pM range, and has 100 pM range when tested on COLO 205 cells [1] .
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay [1]
| Cell Line: | Ramos, Namalwa, HL60/s, and COLO 205 Cancer Cells |
| Concentration: | 0-3 nM |
| Incubation Time: | 72 hours |
| Result: | Inhibited Namalwa and HL60/s cells with IC 50 s of 7 and 30 pM, respectively. |
Appearance
Solid
Shipping
Room temperature in continental US; may vary elsewhere.
Storage
-20°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
DMSO : 50 mg/mL ( 90.91 mM ; Need ultrasonic)
Stock Solutions
| Concentration Solvent Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 1.8181 mL | 9.0907 mL | 18.1815 mL |
| 5 mM | 0.3636 mL | 1.8181 mL | 3.6363 mL |
| 10 mM | 0.1818 mL | 0.9091 mL | 1.8181 mL |
