[CAS NO. 667463-62-9] BIO

Name: BIO
Brand: Arctom Scientific
SKU: SLK-S7198

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PRODUCTS SPECIFICATIONS [667463-62-9]

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Catalog

SLK-S7198

Brand

Selleck

CAS

667463-62-9

DESCRIPTION [667463-62-9]

Overview

MDL	MFCD08705318
Molecular Weight	356.17
Molecular Formula	C16H10BrN3O2
SMILES	O=C1\C(\C=2C(N1)=CC(Br)=CC2)=C/3\C(=N\O)\C=4C(N3)=CC=CC4

For research use only.

Storage

3 years,-20°C,powder
1 years,-80°C,in solvent

Shipping

Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Concentration Volume Mass	1 mg	5 mg	10 mg
1 mM	2.8076 mL	14.0382 mL	28.0765 mL
5 mM	0.5615 mL	2.8076 mL	5.6153 mL
10 mM	0.2808 mL	1.4038 mL	2.8076 mL
50 mM	0.0562 mL	0.2808 mL	0.5615 mL

Description

BIO (GSK-3 Inhibitor IX, 6-bromoindirubin-3-oxime, 6-Bromoindirubin-3'-oxime, MLS 2052) is a specific inhibitor of with of 5 nM for GSK-3α/β in a cell-free assay, shows >16-fold selectivity over CDK5, also a pan- inhibitor with IC50 of 30 nM for . BIO induces in human melanoma cells.

Features

The first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.

Targets

In vitro

BIO (6-bromoindirubin-3'-oxime) is a specific inhibitor of glycogen synthase kinase-3 (GSK-3), with IC50 of 5 nM for GSK-3α/β, shows >16-fold selectivity over CDK5. BIO interacts within the ATP binding pocket of these kinases, reduces β-catenin phosphorylation on a GSK-3-specific site in cellular models, closely mimicks Wnt signaling in Xenopus embryos. In human and mouse embryonic stem cells, BIO maintains the undifferentiated phenotype and sustains expression of the pluripotent state-specific transcription factors Oct-3/4, Rex-1 and Nanog. BIO-mediated Wnt activation is functionally reversible, as withdrawal of the compound leads to normal multidifferentiation programs in both human and mouse embryonic stem cells. BIO promotes proliferation in mammalian cardiomyocytes. 6BIO is also a pan-JAK inhibitor, with IC50 values of 0.03, 1.5, 8.0, 0.5 μM for TYK2, JAK1, JAK2 and JAK3. BIO selectively inhibits phosphorylation of STAT3 and induces apoptosis of human melanoma cells.

In vivo

BIO suppresses melanoma tumor growth in a mouse xenograft model.

References

[1] Meijer L, et al. Chem Biol, 2003, 10(12), 1255-1266.
[2] Sato N, et al. Nat Med, 2004, 10(1), 55-63.
[3] Tseng AS, et al. Chem Biol, 2006, 13(9), 957-963.
[4] Liu L, et al. Cancer Res. 2011 Jun 1;71(11):3972-3279.

Synonyms

2H-Indol-2-one, 6-bromo-3-[(3E)-1,3-dihydro-3-(hydroxyimino)-2H-indol-2-ylidene]-1,3-dihydro-, (3Z)-

(3Z)-6-Bromo-3-[(3E)-1,3-dihydro-3-(hydroxyimino)-2H-indol-2-ylidene]-1,3-dihydro-2H-indol-2-one

(2′Z,3′E)-6-Bromoindirubin-3′-oxime

GSK 3 Inhibitor IX

GSK 3 IX

MLS 2052

BIO

6-Bromoindirubin-3′-oxime

6-BIO

KIN 001-043

(2Z,3E)-6′-Bromo-3-(hydroxyimino)-[2,3′-biindolinyliden]-2′-one