[CAS NO. 193149-74-5] WST-8
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PRODUCTS SPECIFICATIONS [193149-74-5]
Store
Catalog
SLK-S7764
Brand
Selleck
CAS
193149-74-5
DESCRIPTION [193149-74-5]
Overview
| MDL | MFCD09264687 |
|---|---|
| Molecular Weight | 600.47 |
| Molecular Formula | C20H13N6NaO11S2 |
| SMILES | [O-]S(C1=CC(S([O-])(=O)=O)=CC=C1C2=NN(C(C(OC)=C3)=CC=C3[N+]([O-])=O)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)(=O)=O.[Na+] |
For research use only.
Storage
3 years -20°C(in the dark) powder
Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)
Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)
Shipping
Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)
Preparing Stock Solutions
| 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.6654 mL | 8.3268 mL | 16.6536 mL |
| 5 mM | 0.3331 mL | 1.6654 mL | 3.3307 mL |
| 10 mM | 0.1665 mL | 0.8327 mL | 1.6654 mL |
| 50 mM | 0.0333 mL | 0.1665 mL | 0.3331 mL |
Description
WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity that produces corresponding formazan dye that absorbs at 460 nm. WST-8 is typically used as a cell viability indicator in cell proliferation assays.
In vitro
WST-8 is reduced by NADH in good yields at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nm. The formazan is soluble to water at concentrations higher than 0.1 M. WST-8 is useful as a sensitive chromogenic indicator for NADH. It is also applicable to cell proliferation assays as a cell viability indicator.
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