Anti-Zebrafish SEPTIN7A/B Antibody Picoband®
PRODUCTS SPECIFICATIONS
DESCRIPTION
Product Name
Anti-Zebrafish SEPTIN7A/B Antibody Picoband®
SKU/Catalog Number
AZA0A096X788
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Zebrafish SEPTIN7A/B Antibody Picoband® catalog # AZA0A096X788. Tested in WB applications. This antibody reacts with Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.Avoid repeated freezing and thawing.
Cite This Product
Anti-Zebrafish SEPTIN7A/B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA0A096X788)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish SEPTIN7A/B recombinant protein (Position: M1-K348).
Reactive Species
AZA0A096X788 is reactive to SEPTIN7A/B in Zebrafish
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Background of SEPTIN7A/B
Predicted to enable GTPase activity and molecular adaptor activity. Acts upstream of or within defense response to bacterium. Predicted to be located in several cellular components, including cleavage furrow; kinetochore; and midbody. Predicted to be part of septin complex. Predicted to be active in cell division site; microtubule cytoskeleton; and septin ring. Orthologous to human SEPTIN7 (septin 7).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Applications
AZA0A096X788 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Zebrafish
Validation Images & Assay Conditions
Western blot analysis of SEPTIN7A/B using anti-SEPTIN7A/B antibody (AZA0A096X788).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebrafish head tissue lysates,
Lane 2: whole female zebrafish tissue lysates,
Lane 3: whole male zebrafish tissue lysates,
Lane 4: zebrafish embryo tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SEPTIN7A/B antigen affinity purified polyclonal antibody (AZA0A096X788) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for SEPTIN7A/B at approximately 50 kDa. The expected band size for SEPTIN7A/B is at 50 kDa.
