| SKU | BA1033 |
|---|---|
| Applications | IF, FCM |
| Pack size | 0.5ml |
| Host | E. coli |
| Concentration | 1 mg/ml |
| Specificity | This CY3 conjugated Protein A, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1. |
| Storage | At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protected from light. |
| Shipping | Ships in room temperature. Can also ship with gel ice or dry ice but not necessary. |
| Purity | >99% |
| Contents | 1 mg/ml of CY3 conjugated specific antibody; 0.01M PBS, 50% glycerol. |
| Fluorophore | Amax=554nm; Emax=568nm |
| Form | Concentrated, Liquid |
| Conjugation | Cy3 |
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Immunofluorescence, 1.25-5 μg/mL
Flow Cytometry, 1.25-5 μg/mL
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