Mouse IgG ELISA Kit PicoKine®
PRODUCTS SPECIFICATIONS
DESCRIPTION
Product Name
Mouse IgG ELISA Kit PicoKine®
SKU/Catalog Number
EK0101
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IgG ELISA Kit PicoKine® (96 Tests). Quantitate Mouse IgG in Cell Culture Supernatants, Serum. Sensitivity: 10 ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IgG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0101)
Clonality of Antibodies
See Datasheet for details
Capture/Detection Antibodies
polyclonal antibody from goat|polyclonal antibody from goat
Sensitivity
<10 ng/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK0101 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0101 is reactive to IgG in Mouse samples
Validated Sample Types
Cell Culture Supernatants, Serum.
Application Guarantee
EK0101 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IgG
Immunoglobulin G (IgG) is a type of antibody. Representing approximately 75% of serum antibodies in humans, IgG is the most common type of antibody found in blood circulation.[1] IgG molecules are created and released by plasma B cells. Each IgG antibody has two paratopes.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0101-CAP | Anti-Mouse IgG Pre-coated 96-well strip microplate | 1 |
| EK0101-ST | Mouse IgG Standard | 2 vials, 1 ug/tube |
| EK0101-DA | Mouse IgG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.001 | 0.121 | 0.21 | 0.375 | 0.684 | 1.145 | 1.628 | 2.038 |
Data Example Images
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IgG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 2827 | 9440 | 46337 | 3051 | 7802 | 41350 |
| Standard deviation | 144.46 | 446.51 | 2706.08 | 226.99 | 183.35 | 1877.29 |
| CV (%) | 5.1% | 4.7% | 5.8% | 7.4% | 2.4% | 4.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IgG in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2827 | 2502 | 1822 | 1646 | 2199 | 166.48 | 7.6% |
| Sample 2 | 9440 | 9016 | 9079 | 12073 | 9902 | 370.33 | 3.7% |
| Sample 3 | 46337 | 28630 | 42589 | 36320 | 38469 | 2281.21 | 5.9% |
