Mouse TNF beta ELISA Kit PicoKine®
PRODUCTS SPECIFICATIONS
DESCRIPTION
Product Name
Mouse TNF beta ELISA Kit PicoKine®
SKU/Catalog Number
EK0585
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TNF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Lta in cell culture supernatants, serum and plasma (heparin, EDTA or citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNF beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0585)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: L34-L202
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0585 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0585 is reactive to Lta in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA or citrate).
Application Guarantee
EK0585 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Lta
The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0585-CAP | Anti-Mouse Lta Pre-coated 96-well strip microplate | 1 |
| EK0585-ST | Mouse Lta Standard | 2 vials, 10 ng/tube |
| EK0585-DA | Mouse Lta Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.035 | 0.081 | 0.140 | 0.283 | 0.525 | 1.036 | 1.468 | 2.011 |
Data Example Images
Mouse TNF Beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Lta, Dilution ratio of 1:10, concentration in serum and plasma is 2.4-11.9 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNF beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 91 | 379 | 1647 | 85 | 460 | 1810 |
| Standard deviation | 1.83 | 22.10 | 98.33 | 2.48 | 29.30 | 110.05 |
| CV (%) | 2.0% | 5.8%% | 6.0% | 2.9% | 6.4% | 6.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Lta in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 91 | 106 | 93 | 87 | 94 | 2.13 | 2.3% |
| Sample 2 | 379 | 322 | 301 | 352 | 339 | 18.35 | 5.4% |
| Sample 3 | 1647 | 1895 | 1224 | 1470 | 1559 | 46.15 | 3.0% |
