Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit
PRODUCTS SPECIFICATIONS
DESCRIPTION
Product Name
Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0369
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human HGF in cell culture supernatants, serum and plasma (EDTA, citrate). Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0369)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q32-R494 (alpha) & V495-S728 (beta)
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<5 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0369 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0369 is reactive to HGF in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA, citrate).
Application Guarantee
FEK0369 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of HGF
Hepatocyte growth factor (HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass (Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0369-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0369-ST | Human HGF Standard | 2 | 10 ng/tube |
| FEK0369-A | Human HGF Antibody A | 1 | 3ml |
| FEK0369-B | Human HGF Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.014 | 0.081 | 0.116 | 0.217 | 0.357 | 0.631 | 1.261 | 2.033 |
Data Example Images
Human HGF PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human HGF/Hepatocyte growth factor PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 169 | 1246 | 4183 | 168 | 1364 | 4239 |
| Standard deviation | 9.8 | 87.22 | 267.71 | 9.91 | 118.66 | 313.68 |
| CV (%) | 5.8% | 7% | 6.4% | 5.9% | 8.7% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of HGF in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 169 | 159 | 165 | 152 | 161 | 6.41 | 3.9% |
| Sample 2 | 1246 | 1103 | 1132 | 1240 | 1180 | 63.61 | 5.3% |
| Sample 3 | 4183 | 3905 | 4308 | 3657 | 4013 | 252.15 | 6.2% |
