Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit
PRODUCTS SPECIFICATIONS
DESCRIPTION
Product Name
Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0393
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0393)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V117-S268
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0393 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0393 is reactive to IL1B in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK0393 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL1B
The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0393-CAP | Anti-Rat IL1B Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| -ST | Rat IL1B Standard | 2 | 10 ng/tube |
| FEK0393-H | HRP Linked Anti-Rat IL1B Antibody | 1 | 6ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.031 | 0.079 | 0.146 | 0.207 | 0.338 | 0.569 | 1.051 | 1.829 |
Data Example Images
Human ANGPTL2 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-1 Beta/IL-1F2/IL1B PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 58 | 233 | 962 | 62 | 241 | 881 |
| Standard deviation | 3.65 | 11.41 | 69.26 | 4.21 | 12.29 | 68.71 |
| CV (%) | 7.8% | 4.9% | 7.2% | 6.8% | 7.8% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL1B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 58 | 59 | 57 | 56 | 57 | 1.11 | 1.9% |
| Sample 2 | 233 | 268 | 237 | 251 | 247 | 13.71 | 5.5% |
| Sample 3 | 962 | 826 | 929 | 964 | 920 | 56.16 | 6.1% |
