Mouse MCP-1 PicoKine® Quick ELISA Kit
PRODUCTS SPECIFICATIONS
DESCRIPTION
Introduction
Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.
Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol
Product Name
Mouse MCP-1 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0568
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MCP-1 PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Mouse MCP-1 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0568)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: Q24-R96
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<6 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0568 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0568 is reactive to CCL2 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
FEK0568 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL2
Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0568-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0568-ST | Mouse CCL2 Standard | 2 | 10 ng/tube |
| FEK0568-A | Mouse CCL2 Antibody A | 1 | 3ml |
| FEK0568-B | Mouse CCL2 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.014 | 0.051 | 0.090 | 0.193 | 0.406 | 0.726 | 1.465 | 1.897 |
Data Example Images
Human Caspase 3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MCP-1 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 39 | 224 | 394 | 40 | 231 | 398 |
| Standard deviation | 1.56 | 10.75 | 27.18 | 2 | 12.47 | 27.46 |
| CV (%) | 4% | 4.8% | 6.9% | 5% | 5.4% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 39 | 36 | 34 | 34 | 35 | 2.04 | 5.8% |
| Sample 2 | 224 | 228 | 228 | 219 | 224 | 3.69 | 1.6% |
| Sample 3 | 394 | 375 | 431 | 394 | 398 | 20.3 | 5.1% |
