Human CD97 PicoKine® Quick ELISA Kit
PRODUCTS SPECIFICATIONS
DESCRIPTION
Product Name
Human CD97 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1402
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD97/ADGRE5 PicoKine® Quick ELISA Kit (90 minues, 96 Tests). Quantitate Human ADGRE5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD97 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1402)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q21-Q398
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1402 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1402 is reactive to ADGRE5 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
FEK1402 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ADGRE5
Cluster of differentiation 97 is a protein also known as BL-Ac [F2] encoded by the ADGRE5 gene. The CD97 gene is mapped to 19p13.2-p13.12 by fluorescence in situ hybridization. In the immune system, CD97 is known as a critical mediator of host defense. Upon lymphoid, myeloid cells and neutrophil activation, CD97 is upregulated to promote adhesion and migration to sites of inflammation. Moreover, it has been shown that CD97 regulates granulocyte homeostasis. CD97 is expressed at the sarcoplasmic reticulum and the peripheral sarcolemma in skeletal muscle. However, lack of CD97 only affects the structure of the sarcoplasmic reticulum, but not the function of skeletal muscle.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1402-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1402-ST | Human ADGRE5 Standard | 2 | 10 ng/tube |
| FEK1402-A | Human ADGRE5 Antibody A | 1 | 3ml |
| FEK1402-B | Human ADGRE5 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.004 | 0.102 | 0.175 | 0.333 | 0.559 | 1.021 | 1.672 | 2.054 |
Data Example Images
Human R-Spondin-1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD97 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 192 | 1210 | 5765 | 193 | 1108 | 6274 |
| Standard deviation | 12.09 | 62.92 | 253.66 | 13.12 | 77.56 | 288.6 |
| CV (%) | 7% | 5.2% | 4.4% | 6.8% | 7% | 4.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ADGRE5 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 192 | 169 | 180 | 190 | 182 | 9.14 | 5% |
| Sample 2 | 1210 | 1172 | 1113 | 1111 | 1151 | 41.72 | 3.6% |
| Sample 3 | 5765 | 6054 | 5419 | 5224 | 5615 | 318.79 | 5.6% |
